EuGene: maximizing synthetic gene design for heterologous expression
نویسندگان
چکیده
منابع مشابه
EuGene: maximizing synthetic gene design for heterologous expression
UNLABELLED Numerous software applications exist to deal with synthetic gene design, granting the field of heterologous expression a significant support. However, their dispersion requires the access to different tools and online services in order to complete one single project. Analyzing codon usage, calculating codon adaptation index (CAI), aligning orthologs and optimizing genes are just a fe...
متن کاملCombined protein construct and synthetic gene engineering for heterologous protein expression and crystallization using Gene Composer
BACKGROUND With the goal of improving yield and success rates of heterologous protein production for structural studies we have developed the database and algorithm software package Gene Composer. This freely available electronic tool facilitates the information-rich design of protein constructs and their engineered synthetic gene sequences, as detailed in the accompanying manuscript. RESULTS...
متن کاملIntegrating Vectors for Heterologous Gene Expression
A novel shuttle integration cosmid vector (pTOYAMAcos), based on pKU402, and shuttle integration vectors (pTYM18 and pTYM19) were constructed for the cloning of actinomycete DNA and its heterologous expression. These vectors contain oriT of an IncP transmissible plasmid in order to transfer genes by conjugation from Escherichia coli to actinomycetes, and they also contain int derived from actin...
متن کاملDesign Parameters to Control Synthetic Gene Expression in Escherichia coli
BACKGROUND Production of proteins as therapeutic agents, research reagents and molecular tools frequently depends on expression in heterologous hosts. Synthetic genes are increasingly used for protein production because sequence information is easier to obtain than the corresponding physical DNA. Protein-coding sequences are commonly re-designed to enhance expression, but there are no experimen...
متن کاملMetabolic load and heterologous gene expression.
The expression of a foreign protein(s) in a recombinant host cell or organism often utilizes a significant amount of the host cell's resources, removing those resources away from host cell metabolism and placing a metabolic load (metabolic drain, metabolic burden) on the host. As a consequence of the imposed metabolic load, the biochemistry and physiology of the host may be dramatically altered...
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ژورنال
عنوان ژورنال: Bioinformatics
سال: 2012
ISSN: 1367-4803,1460-2059
DOI: 10.1093/bioinformatics/bts465